Research Articles
ddPCR for HIV Co-receptor Mutations: A Superior Approach for Quantifying CCR5Δ32 in Research and Drug Development
This article provides a comprehensive analysis of droplet digital PCR (ddPCR) for detecting and quantifying key HIV co-receptor mutations, with a focused evaluation of its performance in assessing the CCR5Δ32...
Digital PCR and Viral Outgrowth: Correlating Assays to Reshape HIV Cure Assessment
Accurately quantifying the replication-competent HIV reservoir is a central challenge in developing a cure.
Strategies for Enhancing ddPCR Precision in Heterogeneous Cell Analysis: From Assay Design to Clinical Validation
This article provides a comprehensive guide for researchers and drug development professionals seeking to optimize droplet digital PCR (ddPCR) for the analysis of heterogeneous cell populations.
Automated ddPCR Data Analysis Pipeline for CCR5 Allele Quantification: A Comprehensive Guide for Researchers and Drug Developers
This article provides a comprehensive guide for developing and implementing an automated droplet digital PCR (ddPCR) pipeline for the precise quantification of CCR5 alleles, including the CCR5Δ32 mutant.
Strategies for Minimizing Off-Target Effects in CRISPR-Cas9 CCR5 Gene Editing: From gRNA Design to Clinical Validation
This comprehensive review addresses the critical challenge of off-target effects in CCR5 gene editing for HIV therapy, synthesizing current methodologies for detection, quantification, and mitigation.
Eliminating Genomic DNA Contamination: A Comprehensive Guide to Purity in cfDNA ddPCR Analysis
Accurate quantification of circulating cell-free DNA (cfDNA) by droplet digital PCR (ddPCR) is critically dependent on the effective removal of contaminating genomic DNA (gDNA).
Strategies for Minimizing False Positives in ddPCR-Based CCR5Δ32 Detection: A Guide for Research and Drug Development
Accurate detection and quantification of the CCR5Δ32 mutation is crucial for advancing HIV cure strategies, including the evaluation of stem cell transplants and gene-edited therapies.
Optimizing ddPCR for Ultrasensitive CCR5Δ32 Detection: A Guide for HIV Cure Research and Clinical Diagnostics
The accurate quantification of low-frequency CCR5Δ32 variants is paramount for advancing HIV cure strategies, particularly in monitoring the engraftment of edited hematopoietic stem cells or tracking naturally occurring mutants.
Multiplex Droplet Digital PCR Assay Design for CCR5 Genotyping: From Foundational Principles to Clinical Validation
This comprehensive guide details the development and implementation of multiplex droplet digital PCR (ddPCR) assays for precise discrimination between CCR5 wild-type and Δ32 alleles.
Digital PCR as a Precision Tool for HIV Reservoir Quantification and Cure Monitoring Post-CCR5Δ32/Δ32 HSCT
This article examines the critical role of digital PCR (dPCR) in quantifying the HIV reservoir and validating cure strategies, with a specific focus on patients undergoing CCR5Δ32/Δ32 allogeneic hematopoietic stem...