Research Articles
Solving the Knock-In Challenge: A Comprehensive Guide to Boosting CRISPR Efficiency in Primary Cell Cultures
Achieving high-efficiency CRISPR-mediated knock-in in primary cells remains a significant bottleneck in biomedical research and therapeutic development.
Cas9 Nickase Variants: Engineering Single-Strand Breaks for Precision Genome Editing
This article provides a comprehensive resource for researchers and drug development professionals on the application of Cas9 nickase (Cas9n) variants for precision genome engineering.
CRISPR-Cas9 vs. ZFNs and TALENs: A Comprehensive Analysis of Efficiency and Clinical Trial Applications
This article provides a systematic comparison of three major genome-editing technologies—CRISPR-Cas9, ZFNs, and TALENs—focusing on their efficiency, specificity, and clinical trial performance.
Prime Editing: A Guide to Precise Genetic Repair Without Double-Strand Breaks
This article provides a comprehensive overview of prime editing, a revolutionary genome-editing technology that enables precise correction of genetic mutations without introducing double-strand DNA breaks.
TKIT Guides: A Precision CRISPR Strategy for Endogenous Protein Tagging and Knock-In
Targeted Knock-In with Two (TKIT) guides represents a significant advance in CRISPR/Cas9-based genome editing, specifically designed to overcome the challenges of precise DNA integration in hard-to-edit cells like neurons.
Optimizing Homology-Directed Repair Template Design for Precise Gene Correction: A Strategic Guide for Researchers
This article provides a comprehensive guide for researchers and drug development professionals on designing effective homology-directed repair (HDR) templates for precise gene editing.
Harnessing DNA Repair: How CRISPR-Cas9 Double-Strand Break Mechanisms Are Revolutionizing Genetic Disease Therapy
This article provides a comprehensive analysis of the cellular DNA repair mechanisms that determine the success of CRISPR-Cas9 gene editing for genetic diseases.
Fetal Hemoglobin Reactivation: From Genomic Mechanisms to Next-Generation Therapies for Sickle Cell and Thalassemia
This article provides a comprehensive analysis of fetal hemoglobin (HbF) reactivation as a therapeutic strategy for β-hemoglobinopathies.
X-ray Crystallography vs. Cryo-EM: A Structural Biologist's Guide to Choosing the Right Tool
This article provides a comprehensive comparison of X-ray crystallography and cryo-electron microscopy (cryo-EM) for researchers and drug development professionals.
Validating Ligand Binding in Electron Density Maps: A Guide to Methods, Challenges, and Best Practices
Accurately identifying and validating small-molecule ligands in experimental density maps from techniques like X-ray crystallography and cryo-electron microscopy (cryo-EM) is a critical yet challenging step in structure-guided drug design.